Effective pretreatment is essential for producing fermentable sugars from lignocellulosic biomass. In this study, mixed-acid and organic-amine pretreatments were conducted for corncob deconstruction, component fractionation, lignin removal, and enzymatic saccharification. The sulfuric acid/maleic acid system (1:1, w/w)...
Jing-Liang Xu, Hui-Min Cao, Zhaoyang Ju et al.· Biofuel Research Journal· 0 citations
Methanol is a promising renewable C1 feedstock for sustainable single‐cell protein (SCP) production. However, its inherent cytotoxicity and metabolic trade‐offs between cell growth and protein synthesis remain significant bottlenecks. Here, we established an “evolutionary‐rational” dual‐driven paradigm to construct a h...
Chong Xie, Cheng-Chao Zhu, Jun-Ze Liu et al.· Biotechnology Journal· 0 citations
Developing sustainable single-cell protein (SCP) from non-food feedstocks offers a promising strategy to address the escalating global demand for sustainable nutrition. However, the lack of industrially robust platforms capable of cost-efficient multi-substrate assimilation remains a key bottleneck. Here, we discover...
D-Amino acid oxidase (DAAO) catalyzes the oxidation of D-phosphinothricin (D-PPT) to produce optically pure L-PPT, but low catalytic efficiency limits its industrial application. Here, a synergistic strategy combining geometric remodeling and charge engineering was applied to enhance the oxidation activity of our previ...
Meng-Yu Li, Chen-Chen Fu, W. Zhuang et al.· Journal of Agricultural and...· 0 citations
Spirulina is recognized as a nutrient-dense superfood. Recirculation of cultivation medium offers a sustainable and cost-effective approach for microalgae production. However, the present study revealed that the Spirulina biomass yield in the second recycled medium (R2 batch) in shake flasks was 70.8% lower than that i...
This study systematically elucidates the synergistic lignin conversion mechanism of CDGs by a fungal consortium from structural, enzymatic, and metabolic perspectives, uncovering a temporal division of labor together with new lignin catabolic pathways that provide a mechanistic framework for the biological valorization...
