Jinghua Yu

Dual-dynamic control and metabolic rebalancing guided by toxic target discovery for efficient dencichine production

Construction of microbial cell factories often requires extensive reconfiguration of metabolic networks, which frequently compromises cell growth. Here, we engineered E. coli to produce dencichine (β-ODAP), a plant-derived hemostatic agent. Knocking out serB blocks the competing L-serine pathway, causing L-serine auxot...