Global transcriptional rewiring and dual-compartment engineering for efficient 3-hydroxypropionic acid production in Saccharomyces cerevisiae.
This work establishes a generalizable framework for multi-compartment malonyl-CoA utilization in eukaryotic cell factories through dynamic control of HFA1 and optimized POS5 expression, and develops a dual-compartment coordination strategy to efficiently exploit cytosolic and mitochondrial malonyl-CoA pools.