John J. Evans

Structural basis of end processing in the nucleosome by polynucleotide kinase phosphatase

Cryo-EM structures of PNKP engaged with non-ligatable SSBs at three unique positions within the nucleosome reveal that PNKP locally deforms nucleosomal DNA to reposition the SSBs into the kinase and phosphatase active sites, providing a structural basis for the efficient processing of SSBs throughout the nucleosome.