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It is indicated that spike protein mutations are involved in the attenuation of GVI-1 IBV strains, and insights into the molecular basis of IBV attenuation during serial passage are provided.
A chimeric subunit vaccine, RBD-HA, in which the receptor-binding domain of the QX-type IBV spike protein replaced the immunodominant head domain of H9N2 AIV haemagglutinin is developed, providing proof of concept for a bivalent subunit vaccine targeting two major avian respiratory viruses.
This study is the first to identify circ-INSR as a host factor contributing to influenza virus replication, and provides a basis for developing circRNA-based strategies against H9N2 avian influenza virus.
These findings establish ckATP1A1-AS1 as a key antiviral lncRNA that restricts IAV replication by coordinating innate immune signaling and directly targeting several steps in the viral replication cycle.
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