Jun-Hong Jiang

Protocol for identifying glycosylated RNAs using solid-phase chemical conjugation and glycosidase release

Summary RNA glycosylation plays a critical role in cellular signaling. Here, we present a protocol for enriching glycoRNAs from cultured cells and clinical tissues using a solid-phase chemoenzymatic technique. We describe steps for employing galactose oxidase (GAO) to selectively oxidize terminal sugar residues, enabli...