BBX transcription factors can respond to UV-B signals, promote plant photomorphogenesis, and regulate plant anthocyanin biosynthesis. Based on transcriptome sequencing analysis, this study analyzed the transcription factors of StBBXs under UV-B treatment and identified two differentially expressed genes, StBBX19 and StBBX24. We explored the protein structures, promoters, and phylogenetics of StBBX19 and StBBX24, speculating that they respond to UV-B treatment and regulate anthocyanin biosynthesis. We constructed overexpression vectors, YFP, and bimolecular fluorescence complementation vectors for the transient transformation of tobacco. The results suggest that StBBX19 and StBBX24 may not function as direct regulators of anthocyanin biosynthesis, but rather enhance anthocyanin accumulation through interaction with StMYB308. Still, after interacting with StMYB308, purple spots appeared on tobacco leaves, suggesting that the interaction between StBBX19, StBBX24, and StMYB308 increases anthocyanin content in tobacco leaves. This study provides significant evidence for the adaptation mechanism of plants under UV-B treatment and offers new insights for molecular plant breeding of pigmented potatoes.
Quiju Dong, Xinyu Liu, Jun Li et al.· BMC Plant Biology· 0 citations
Fusarium proliferatum is a postharvest pathogen responsible for severe rot in economically important fruits and vegetables, leading to food waste and safety concerns due to mycotoxin contamination. While the PHO signaling pathway is known to regulate phosphate homeostasis, its specific contribution to the virulence mechanisms of this foodborne pathogen remains unclear. In this study, we functionally characterized key components of the PHO pathway in F. proliferatum. Targeted deletion of PHO pathway genes (FpNuc1α, FpNuc1β, FpNuc2, FpPho80, and FpPho85) revealed their essential roles in vegetative growth, conidiation, and stress responses critical for surviving in storage environments. Transcription factor FpNuc1α is required for the full virulence of F. proliferatum. We demonstrate that FpNuc1α activity is tightly controlled by a phosphorylation switch in response to phosphate availability. Furthermore, we discovered that FpNuc1α recognizes a novel DNA motif (BSN) to directly activate FpGit1 gene, a glycerophosphodiester transporter required for full virulence. These findings uncover a specific regulatory mechnism linking phosphate sensing to the infection process of F. proliferatum, providing potential molecular targets for developing novel fungicides to control postharvest decay and ensure food safety.
Yizhou Gao, Yi-Tong Wang, Zenghui Yang et al.· Food microbiology· 0 citations
