A Rapid and Field-Compatible Detection Platform for the AvrPi9 Gene in Magnaporthe oryzae Using Integrated DNA Extraction, RPA and CRISPR–Cas12a
Rice blast, caused by Magnaporthe oryzae, remains one of the most destructive diseases of rice worldwide. Although molecular diagnostics enable rapid pathogen detection, tools for monitoring avirulence genes that directly inform resistance deployment remain limited. Here, we developed a field-compatible molecular detec...