A field deployable duplex RPA-CRISPR/Cas12a assay for rapid and sensitive detection of African swine fever virus
A rapid, field-deployable recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay for the detection of African swine fever virus using both fluorescence- and lateral flow strip (LFS)-based readouts shows 100% analytical specificity against other porcine viral pathogens and strong agreement with real-time PCR.