Background: The oxidative stress caused by diabetes mellitus (DM) damages hepatocytes and reduces liver function. In this context, flavonoids and acetogenins found in soursop (Annona muricata L.) leaf have hepatoprotective and antioxidant qualities. Therefore, this study aimed to assess the effect of Annona muricata leaf extract on hepatocyte index and hepatic histopathology in diabetic Wistar rats.Methods: In a laboratory experiment, 16 male Wistar rats weighing 100–150 g and aged 2-3 months were given a high-fat diet and an injection of streptozotocin (27.5 mg/kg BW) to induce type 2 diabetes. Rats were placed in a randomized posttest-only control group. For 21 days, these experimental animals were split into two groups at random, namely control (n = 8) receiving aquadest, and treatment group (n = 8) receiving Annona muricata leaf extract at a dose of 100 mg/kg BW. Hematoxylin-eosin staining was used to analyze hepatic histopathology, and a quantitative evaluation of hepatocyte index was conducted. The independent t-test and Mann-Whitney U test were adopted to assess the data. Results: There was a significant difference (p=0.041) in hepatocyte index between treatment and control groups. Treatment group had less hepatocyte deterioration than the control, according to histopathological scoring (p=0.037).Conclusion: Diabetic Wistar rats treated with Annona muricata leaf extract showed better hepatocyte index and decreased hepatic degeneration, suggesting hepatoprotective potential
Dewa Ayu Agung Alit Suka Astini, Desak Putu Oki Lestari, L. G. Evayanti et al.· The Indonesian Journal of Ga...· 0 citations
Abstract Objective Epilepsy is a neurological disorder characterized by recurrent spontaneous seizures due to excessive brain electrical activity. Identifying factors associated with seizure recurrence may help improve patient's management. This study aims to identify factors associated with recurrent spontaneous seizures in epilepsy patients. Method This study was an analytical cross-sectional study with consecutive samples of epilepsy patients at Sanjiwani Gianyar Regional General Hospital. Data were collected through structured patient interviews and review medical records, capturing demographic characteristics, clinical history, and seizure-related variables. Samples were classified into two groups based on the presence or absence of seizure recurrence within the past year. Comparative analysis between groups was conducted using the Chi-square test. Results A total of 41 samples were obtained: 21 from the group without seizures and 20 from the group with seizures. Of several variables, those associated with recurrent spontaneous seizures were electroencephalography (EEG) results ( p = 0.012), history of status epilepticus ( p = 0.048), seizures during sleep ( p = 0.020), and recurrent seizures within the first 3 months after the first seizure ( p <0.001). Conclusion Recurrent spontaneous seizures in epilepsy patients are associated with EEG findings, history of status epilepticus, seizures during sleep, and recurrent seizures within the first 3 months after the first seizure. Within the limitations of this study, EEG abnormalities appear to be associated with seizure recurrence. These findings are still preliminary and should be interpreted with caution.
S. Harkitasari, K. Sumadewi, Ida Bagus et al.· International Journal of Epi...· 0 citations
