Clinical evaluation of an in-house probe-based real-time PCR assay for early diagnosis of leptospirosis compared with Microscopic Agglutination Test
Introduction: TaqMan probe-based real-time PCR assay targeting the lipL32 gene offer higher specificity and accuracy than conventional PCR for leptospirosis diagnosis. However, it has not been implemented in Sri Lanka.Methods: An in-house real-time PCR assay targeting lipL32 gene of pathogenic Leptospira species was d...