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Sponsored AI Networking Cookbook: Practical recipes for AI-assisted network automation and development AI Networking Cookbook: Practical recipes for AI-assisted network automation and development

Author

Katsuhiko S. Murakami

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Open access Aug 2026

Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster

This study shows that native Pol II transcription complexes are isolated directly from Drosophila melanogaster embryos by using a robust one-step affinity purification and by using cryo-EM resolve multiple functional states of the Pol II transcription complex and nucleosome.

Natalie L. Venette-Smith, R. Vishwakarma, Varun Venkatakrishnan et al. · 0 citations
Open access Aug 2026

Clamp conformational flexibility and dynamics in archaeal and eukaryotic RNA polymerases revealed by cryo-EM

Cryo-electron microscopy is addressed using cryo-EM to examine archaeal RNAPs from Euryarchaeota and Crenarchaeota alongside yeast RNAP II and suggests that evolutionary tuning of clamp flexibility and dynamics contributes to distinct GTF-dependent promoter-loading mechanisms.

George Nkansah Rost Fordjour, L. Palao, Kenji Murakami et al. · 0 citations
Open access Jul 2026

Cryo-EM study of bacteriophage N4 virion RNA polymerase

Cryo-electron microscopy structures of full-length N4 vRNAP in its apo state and in a transcription initiation complex with promoter DNA and initiating nucleotides are reported, revealing how the C-terminal domain regulates polymerase activity through a structural “switch” that locks the enzyme in an inactive state unt...

M. Narwal, Y. Shin, Katsuhiko S. Murakami · 0 citations

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