Multi-Enzyme Peptide Mapping of Site-Selective Trastuzumab Modification by an Engineered Thermostable Microbial Transglutaminase
Peptide mapping by liquid chromatography-tandem mass spectrometry (LC–MS/MS) is a key analytical strategy for residue-level characterization of antibody conjugates, providing modification-site assignments and apparent relative modification estimates that complement intact-mass and chromatographic drug-to-antibody rat...