Ke-Ye Xu

A 6His-2Flag dual-tag strategy enables high-yield production of antimicrobial peptide N6 in Pichia pastoris to combat multidrug-resistant Klebsiella pneumonia

In response to the global threat posed by multidrug-resistant Klebsiella pneumoniae, this study established an efficient heterologous expression system for the antimicrobial peptide N6 in Pichia pastoris X-33. By employing an innovative 6His-2Flag dual-tag fusion strategy coupled with formic acid-mediated cleavage, we...