Rapid purity and charge-variant analysis of bispecific antibodies using microfluidic capillary electrophoresis-mass spectrometry.
The usefulness of microfluidic capillary electrophoresis-mass spectrometry (MCE-MS) for rapid purity and proteoform profiling of BsAb samples is demonstrated and the developed MCE-MS workflow exhibits great potential to accelerate and enhance decision-making during BsAb development and production.