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Open access Aug 2026

Development and Validation of an Antigen-Capture ELISA for Quantitative Detection of Donkey-Origin Rotavirus: Application in Vaccine Manufacturing Process Monitoring

Rotavirus A (RVA) causes severe diarrhea in donkey foals, but rapid quantitative antigen assays for vaccine development and manufacturing control are lacking. We developed a VP6-based antigen-capture ELISA (acELISA) for donkey-origin RVA using rabbit anti-RVA polyclonal IgG as the capture antibody and horseradish perox...

Ze-Nan Zhang, Xing Guo, Kui Guo et al. · 0 citations

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