Enzymatic synthesis of neotrehalose: Deciphering the β-1 catalytic promiscuity of transglycolytic starch-active enzymes.
Neotrehalose is a naturally occurring, non-reducing, rare disaccharide with promising nutritional, medical, and synthetic applications, but its low natural abundance and complex chemical synthesis limit commercial production. This study evaluated starch-active enzymes as a sustainable route for its synthesis from renew...