Reconstituting the Motility of Intraflagellar Transport Trains Ex Vivo.
Intraflagellar transport (IFT) is essential for the assembly, maintenance and function of most eukaryotic cilia. It acts as a bidirectional cargo transport system, powered by kinesin and dynein motors to navigate through the crowded ciliary environment. Here, we describe a method to isolate intact IFT trains from live...