Le-Tian Tan

Reconstruction and metabolic regulation of O-succinyl-L-homoserine biosynthesis pathway in Escherichia coli W3110.

The plasmid-free system constructed in this study effectively avoids the plasmid-induced metabolic burden and genetic instability and demonstrates the prominent advantages and great application potential of plasmid-free modular engineering for the efficient biosynthesis of OSH and other high-value amino acids.