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Author

Lei Shi

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2 papers indexed here

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Jul 2026

The E3 ligase BnNLA1 modulates seed coat color in Brassica napus through ubiquitination of BnC07MYB3a.

In rapeseed (Brassica napus L.), excessive accumulation of flavonoid pigments (anthocyanins/proanthocyanidins) compromises the nutrition, flavor, and commercial value. Therefore, reducing the accumulation of seed coat pigments is one of the main objectives in B. napus breeding. To elucidate the mechanisms affecting the genetic architecture of seed coat color, a genome-wide association study (GWAS) of seed coat color was conducted with a diverse group of 393 B. napus cultivars. NITROGEN LIMITATION ADAPTATION1 (NLA1) was identified as a previously unrecognized regulator that controls seed coat color and participates in flavonoid biosynthesis and accumulation in B. napus. Increasing evidence suggests that BnNLA1 (BnA09NLA1/BnC08NLA1) interacts with and ubiquitinates BnC07MYB3a, an R2R3-MYB-type transcription factor and a candidate regulator of the seed coat color in B. napus, and the lysine residue K164 of BnC07MYB3a is the key ubiquitination. Both the BnA09NLA1/BnC08NLA1 knock-out (KO) mutants and overexpressing BnC07MYB3a lines in B. napus exhibit lighter seed coat color indicating lower anthocyanin and proanthocyanidin accumulation compared with the wild-type plants. BnC07MYB3a also directly binds to the promoter of the TRANSPARENT TESTA 6 (BnTT6) and BANYULS (BnBAN), and represses their expression in B. napus. As expected, the expression levels of BnTT6 and BnBAN are significantly reduced in the BnA09NLA1/BnC08NLA1 KO mutants compared to the wild-type plants. Our findings reveal a novel regulatory framework mediated by the BnNLA1-BnC07MYB3a module for controlling seed coat color in B. napus and provide a new strategy for breeding high-quality B. napus cultivars.

Ran Hu, Haijiang Liu, Yunshan Tang et al. · 1 citation
Open access Aug 2026

The PHR1-RSL2 module regulates root hair elongation induced by phosphate deficiency.

Phosphorus (P) is an essential macronutrient for plant growth and development. Root hairs enhance P acquisition as inorganic phosphate (Pi) from soil by expanding the root surface area, and their elongation is a key adaptive response to low Pi availability. However, the transcriptional regulators that couple Pi starvation signaling to root hair elongation remain largely unknown. Here, we demonstrate that PHOSPHATE STARVATION RESPONSE1 (PHR1), the central transcription factor of the Pi starvation response, positively regulates Pi deficiency-induced root hair elongation in Arabidopsis. RNA-seq analysis of root tips identified ROOT HAIR DEFECTIVE 6-LIKE 2 (RSL2), a bHLH transcription factor governing root hair elongation, as a prominent PHR1-regulated target. We show that PHR1 binds to the promoter of RSL2 to activate its expression, and genetic analysis confirms that RSL2 acts downstream of PHR1. Further RNA-seq analysis revealed that RSL2 regulates cell wall remodeling genes, among which XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE 26 (XTH26) was identified as a key target. RSL2 binds to the promoter of XTH26 to upregulate its transcription, and XTH26 overexpression partially rescues the reduced root hair length of both phr1 and rsl2. Collectively, our findings delineate a PHR1-RSL2 transcriptional module that orchestrates root hair elongation under Pi deficiency, thereby contributing to enhanced Pi acquisition.

Xinyu Yang, Yi Ding, Yajie Wang et al. · 0 citations