A versatile phenotyping platform combining three-photon microscopy, optimized tissue clearing, and deep-learning signal separation for full-depth multi-organelle phenotyping.
Imaging the internal structure of plant organs at the subcellular level is essential for understanding key processes such as photosynthesis, growth, and stress responses. Yet conventional optical microscopy struggles to visualize deep tissues due to intense light scattering and spectral crosstalk from autofluorescent c...