Liming Liu

Branched-chain amino acid biosynthetic platforms in prokaryotic cell factories: metabolic engineering and value-added chemical biomanufacturing.

Branched-chain amino acids (BCAAs) are recognized not only as industrially important fermentation products but also as versatile biosynthetic platforms for the production of branched-chain value-added chemicals. The characteristic branched carbon skeletons of BCAAs, together with their metabolically versatile intermedi...

[Efficient production of l- lysine by Escherichia coli through systems metabolic engineering].

[Construction of an integrative recombinant Escherichia coli strain for efficient biosynthesis of chondroitin].

Metabolic flux reprogramming and protein engineering drive efficient l-arginine biosynthesis.

l-arginine is widely used in food, feed, pharmaceutical, and cosmetic industries. However, its industrial-scale biosynthesis is limited by insufficient coordination between metabolic regulation, pathway engineering, and fermentation optimization. In this study, an enzyme-constrained model (ec_iML1515) was used to ident...

Systems metabolic engineering of Corynebacterium glutamicum for efficient production of L-isoleucine

L-Isoleucine is an essential branched-chain amino acid for livestock and poultry, supporting protein accretion and regulating energy metabolism, immune function, and stress resilience. A genetically stable L-isoleucine producer, Corynebacterium glutamicum cgl-Ile0, was obtained via biosensor-assisted ARTP mutagenesis,...