Li-Ming Liu

Branched-chain amino acid biosynthetic platforms in prokaryotic cell factories: metabolic engineering and value-added chemical biomanufacturing.

Branched-chain amino acids (BCAAs) are recognized not only as industrially important fermentation products but also as versatile biosynthetic platforms for the production of branched-chain value-added chemicals. The characteristic branched carbon skeletons of BCAAs, together with their metabolically versatile intermedi...

[Efficient production of l- lysine by Escherichia coli through systems metabolic engineering].

[Construction of an integrative recombinant Escherichia coli strain for efficient biosynthesis of chondroitin].

This study provides a robust strategy for developing efficient microbial cell factories for the production of glycosaminoglycans by developing an efficient genome-integrated Escherichia coli strain for chondroitin production based on the strain GZ17 previously constructed in the laboratory.

Metabolic flux reprogramming and protein engineering drive efficient l-arginine biosynthesis.

An enzyme-constrained model was used to identify 11 gene targets affecting l-arginine production and engineered the rate-limiting enzyme argininosuccinate synthetase to construct the strain Arg11, achieving the best performance reported to date.

Systems metabolic engineering of Corynebacterium glutamicum for efficient production of L-isoleucine

L-Isoleucine is an essential branched-chain amino acid for livestock and poultry, supporting protein accretion and regulating energy metabolism, immune function, and stress resilience. A genetically stable L-isoleucine producer, Corynebacterium glutamicum cgl-Ile0, was obtained via biosensor-assisted ARTP mutagenesis,...