Lin Yang

Semi-rational design of an (S)-selective ω-transaminase for the asymmetric synthesis of the bulky aryl-alkyl chiral amine (S)-3-methyl-1-phenylbutylamine.

ω-Transaminases (ω-TAs) provide an efficient route to the asymmetric synthesis of chiral amines. However, the intrinsic steric constraints of native ω-TA active sites lead to extremely low or undetectable catalytic activity toward bulky aryl-alkyl substrates. Here, we combined a substrate-truncation strategy with semi-...