Thaumatin II is a high-intensity sweet protein whose limited thermostability restricts its industrial application. Using the previously evolved high-yield Thaumatin variant M-882 as the parental scaffold, we used an integrated rational design strategy combining HotSpot Wizard, FoldX, and PyRosetta. Candidate variants...
Le-Fei Wang, Yu-Xin Yang, Jin-Tong Dong et al.· Journal of Agricultural and...· 0 citations
Alternative proteins and cultivated meat increase the need for flavour strategies that maintain savoury quality with less reliance on salt and conventional seasonings. Food-derived umami peptides are promising candidates, but their practical value depends on evidence beyond sequence prediction. With advances in virtual...
Chun-Ming Zhou, Yang Sun, Li-Ying Zhu et al.· Foods· 0 citations
Ectoine, a cyclic amino acid derivative synthesized via the aspartate pathway, has emerged as a promising bioactive compound in the functional food industry owing to its exceptional protective effects on cellular structures and macromolecules. Despite its natural synthesis by halophilic microorganisms such as Halomonas...
En-Tong Zhu, Zi-Xuan Tao, Yue-Tong Fu et al.· Food Research International· 0 citations
The enzymatic degradation of poly(ethylene terephthalate) (PET) offers a sustainable route for plastic recycling but is often hindered by limited enzyme adsorption on hydrophobic surfaces. Inspired by carbohydrate-binding modules (CBMs), which enhance enzyme performance on insoluble substrates, we developed a machine-l...
Rui Long, Yaxin Tang, Chengyong Wang et al.· Bioresource Technology· 1 citation
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