Expression, purification, and functional analysis of a recombinant Bacillus tequilensis strain YIV keratinase for green bioprocessing of keratin biomass.
Keratinases are specialized proteases capable of degrading recalcitrant keratin and have attracted considerable interest for environmentally sustainable bioprocessing. In this study, a keratinase gene from Bacillus tequilensis strain YIV, isolated from tannery waste in Kasur, Pakistan, was cloned, expressed, and charac...