Single-gRNA CRISPR/eCas9-mediated simultaneous targeting of cIAP1 and cIAP2 in colorectal cancer cells: RNA-seq reveals distinct transcriptomic and apoptotic responses.
Cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and cIAP2), encoded by BIRC2 and BIRC3, respectively, are important regulators of cellular survival and apoptosis. These proteins contribute to cancer cell survival by suppressing apoptotic signaling. While individual knockouts of BIRC2 or BIRC3 in cancer cells ha...