Fusarium solani is a major soil-borne pathogen responsible for root rot diseases, posing a significant threat to agricultural productivity. The development of environmentally sustainable alternatives to chemical fungicides is therefore urgently needed. This study evaluated the antifungal activity of fermentation broths and extracellular metabolites derived from Tilletia laevis fungi at different developmental stages against F. solani under in vitro conditions. Six test agents, including carbendazim (one positive fungicide control), caffeic acid, phenylacetylglycine, and 6-hydroxypyridine-2-carboxylic acid (candidate bioactive metabolites), were evaluated for mycelial growth inhibition, EC50 values, and physiological responses. All treatments exhibited concentration-dependent inhibitory effects, with carbendazim showing the highest activity (EC50 = 21.26 mg L−1). Among the metabolites, 6-hydroxypyridine-2-carboxylic acid and phenylacetylglycine demonstrated notable antifungal efficacy, particularly at higher concentrations. Fermentation broths from the promycelial stage showed stronger inhibition than those from the teliospore stage, indicating stage-specific metabolite activity. Biochemical analyses of F. solani mycelia revealed significant changes in soluble protein, soluble sugar, and antioxidant enzyme activities (SOD and POD), suggesting that antifungal effects are mediated through metabolic disruption and oxidative stress. These findings highlight the potential of T. laevis-derived metabolites as eco-friendly biofungicides and provide a theoretical basis for sustainable management of soil-borne diseases. Further studies are required to identify active compounds and validate their efficacy under field conditions.
De-Lai Chen, Shirong Ma, Wei-Wei Zhang et al.· Journal of Fungi· 0 citations
Geomorphic features, drainage patterns and topography are key indicators of active tectonics. This study examines geomorphological characteristics and geological hazards, specifically earthquakes and landslides, in the Muzaffarabad region. The findings contribute to disaster risk reduction and achievement of sustainable development goals (SDGs). Field‐based geomorphological studies and satellite imagery were used to analyse the geomorphological characteristics of the region. Five geomorphic indices, namely asymmetry factor (AF), drainage basin shape index (Db), slope analysis, valley floor width‐to‐height (
V
f
) ratios and hypsometric curves, were calculated using shuttle radar topography mission digital elevation model (SRTM DEM). Features such as drainage offsets, stream deflections, faceted spurs, seismicity, deformed recent sediments and point bars dissection near the Jhelum and Muzaffarabad faults suggest left‐lateral oblique‐slip motion, indicating the tectonically active nature of these faults. The geomorphic and neotectonics analysis concludes that the Muzaffarabad region is highly susceptible to earthquakes and landslides, particularly, in fault‐affected areas. A flood susceptibility analysis using multi‐criteria decision analysis (MCDA) in ArcGIS was conducted to enhance climate resilience and disaster preparedness (SDG 13) and support (SDG 6) by identifying flood‐prone areas. Three high‐risk flood zones were identified: Neelum River–Shawai Nala confluence, Jhelum–Neelum rivers convergence at Domel and a low‐lying area near the Lohargali landslide. This study highlights the importance of integrating morphometric, geomorphological and geospatial techniques for effective disaster risk reduction (SDGs 11 and 13), climate‐resilient infrastructure (SDG 9), addressing SDG 6 and sustainable urban development, ensuring long‐term safety in tectonically active regions. Additionally, the methodology in this study, integrating multiple existing techniques, can be expanded for hazard assessment in other regions.
Waqar Ayub, Ahmed Nabi, M. Jabran et al.· Geological Journal· 0 citations
