Introduction: Dengue fever is a climate-sensitive vector-borne disease. While many studies have documented associations between climate variables and dengue incidence, most rely on conventional regression approaches that fail to separate direct and indirect climatic pathways, limiting mechanistic understanding and utility for environmental health. This study aims to elucidate the interconnected effects of climatic variability on dengue incidence in Surabaya. This study integrates seasonal time-series decomposition and path analysis to distinguish direct and indirect climatic pathways, sunlight duration and wind speed. Methods: A retrospective ecological design, monthly dengue incidence data from 2020 to 2024 were integrated with meteorological variables, including rainfall, temperature, humidity, sunlight duration, and wind speed. Seasonal time-series decomposition was applied to characterize temporal dynamics, followed by path analysis within a structural equation modeling framework to quantify direct and indirect climatic effects. Results and Discussion: A pronounced seasonal pattern in dengue incidence, with peaks during the rainy season. Maximum temperature, relative humidity, sunlight duration, and minimum wind speed exerted significant direct effects on dengue incidence (p < 0.05). Rainfall showed no direct association but acted as a key upstream driver influencing other climatic variables. The structural model explained 52.1% of the variance in dengue incidence, highlighting the contribution of interconnected climatic processes. Conclusion: These findings demonstrate that dengue dynamics in tropical metropolitan environments are governed by complex climate-mediated pathways rather than isolated climatic predictors. This study novel environmental epidemiological evidence to support climate-informed dengue early warning systems and adaptive vector control strategies under climate variability and urban environmental change.
K. Diyanah, R. Yudhastuti, L. Sulistyorini et al.· Jurnal kesehatan lingkungan· 0 citations
The genus Dendrobium is a rich source of bioactive secondary metabolites, particularly bibenzyl derivatives with substantial pharmacological activities, but Dendrobium stuartii remains an underexplored species. Therefore, this study aimed to investigate the drug discovery potential of D. stuartii through an integrated in silico and experimental method. Four key compounds were prioritized through literature analysis, and the interactions with the epidermal growth factor receptor (EGFR) were evaluated using molecular docking. The chemical profile of the acetone extract was characterized using Liquid Chromatography–High Resolution Mass Spectrometry (LC-HRMS). Additionally, biological activities were assessed through antibacterial, antibiofilm, and anti-inflammatory assays. Batatasin III had the strongest predicted binding affinity toward EGFR, and the LC-HRMS analysis confirmed the presence of bibenzyl derivative 3,4ʹ-dihydroxy-5,5ʹ-dimethoxybibenzyl (gigantol). The extract provided antibacterial activity, specifically against S. aureus and P. acnes, inhibited biofilm formation during the mid-phase, and produced substantial anti-inflammatory activity, as evidenced by significant inhibition of protein denaturation. The results suggested D. stuartii as a promising underexplored source of bioactive compounds with potential applications in anticancer and anti-infective drug development.
Deni Setiawan, Samsul Hadi, Nur Mahdi et al.· Journal of Applied Pharmaceu...· 0 citations