Open access
Sep 2026
Architecture of a DNA-guided Cas12a.
CRISPR-Cas systems have largely been restricted to RNA-guided nucleases. Here, we present the cryo-electron microscopy structure of Acidaminococcus sp. Cas12a bound to a pseudo-DNA (ΨDNA) guide and RNA target, revealing how Cas12a can accomplish DNA-guided RNA recognition. The ΨDNA hairpin bridges the recognition and n...
R. F. Ocampo, Carlos Orosco, Bo-Yu Huang et al.
· Nature Structural & Molecula... · 0 citations