ABSTRACT Metagenomic next-generation sequencing (mNGS) is increasingly used in infectious disease diagnostics, yet how library preparation shapes the microbial, functional, and host signals recovered from clinical samples remains poorly defined. Here, we performed a within-sample parallel comparison of three mNGS libra...
Geng-Yan Luo, Jin Zhao, Lin-Lin Xie et al.· mSystems· 0 citations
Parasite communities in the internal organs of small mammals remain poorly characterized because metatranscriptomic pathogen discovery has largely focused on viruses and bacteria. We individually sequenced liver transcriptomes from 351 small mammals collected across 14 cities in Guangdong Province, China, and combined...
Min-wu Peng, De Wu, Qing-Quan Cen et al.· bioRxiv· 0 citations
Mosquitoes are pathogen vectors embedded within diverse microbial ecosystems. However, the nature and interactions among their multi-kingdom microbiome remain poorly understood. We conducted a nationwide single-mosquito meta-transcriptomic survey of 5,163 mosquitoes representing 100 species across China, integrating vi...
Qin-yu Gou, Wei-Chen Wu, Pei-Bo Shi et al.· bioRxiv· 0 citations
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