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Author

Megan L. Maple

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Aug 2026

Measuring Live-Cell mRNA Translational Dynamics with Split Luminescent Tagging in HEK293 Cells.

The versatility of the assay is demonstrated and a 4-fold reduction in 5' cap concentration for in vitro transcription results in equivalent translation in cellulo, illustrating how split luminescent tagging can be easily integrated into the mRNA therapeutic workflow, enabling monitoring of real-time mRNA-driven protein expression dynamics in cellulo.

C. Batho, Camilla Ascanelli, Megan L. Maple et al. · 0 citations