Genome-wide CRISPR screen identifies RNF24 as a critical host factor for foot-and-mouth disease virus entry
An unbiased genome-wide CRISPR/Cas9 knockout screening using porcine cells reveals a ubiquitin-dependent RNF24-LPXN regulatory axis that supports FMDV entry, highlights the role of non-degradative ubiquitination in viral pathogenesis, and proposes this interface as a potential target for antiviral intervention.