Author

Michael A. Gapuz

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Open access Aug 2026

Expanding the catabolic capacity of Pseudomonas putida to acetovanillone, 5-carboxyvanillate, and vanillyl glyoxylate for muconate production from kraft lignin-derived aromatics

The pulp and paper industry produces large volumes of condensed kraft lignin, which is challenging to convert to single chemical products. For this purpose, tandem chemical depolymerization and bioconversion to a single atom-efficient product is a potentially promising strategy. In this study, we conducted copper-catal...

Kathryn M. Mains, Dillon T. Hofsommer, Michael A. Gapuz et al. · bioRxiv · 1 citation

Open access Jul 2026

Engineered Pseudomonas putida reconfigures metabolic fluxes to support energy demands during muconate bioproduction from lignin-related aromatics

Pro proteomics, metabolomics, and 13C-fluxomics are applied to quantitatively compare central carbon and energy metabolism in wild-type P. putida and a muconate-producing strain for improved bioproduction from renewable feedstocks.

R. Wilkes, P. Suthers, A. Borchert et al. · bioRxiv · 3 citations

Open access Jul 2026

Overcoming protocatechuate and catechol accumulation in muconic acid production via adaptive laboratory evolution and metabolic engineering in Pseudomonas putida

Results indicate that catechol, not PCA, is the principal bottleneck in muconate production via the PCA decarboxylation route originally demonstrated by Draths et al., refining the understanding of pathway limitations and offering new strategies for improving rate, yield, and strain resilience in muconate bioproduction...

Alissa C. Bleem, Tracy L. Hodges, Torrey M Lind et al. · bioRxiv · 2 citations