Development and fit-for-purpose validation of a qPCR assay for biodistribution assessment of plasmid DNA following electrode-needle device-assisted intramuscular electroporation.
Reliable biodistribution assessment is essential for the nonclinical evaluation of plasmid DNA (pDNA)-based therapeutics. Here, we developed and performed fit-for-purpose validation of a TaqMan-based quantitative PCR (qPCR) assay for the absolute quantification of pDNA following electrode-needle device-assisted intramu...