Ming-Hua Ji

Computational simulation to improve the activity of a heterologous feruloyl esterase secreted by Bacillus subtilis.

Feruloyl esterases (FAEs) release ferulic acid from plant cell walls but suffer from low efficiency and poor stability. A putative FAE (FAE-BSaf) from Bacillus safensis was expressed in Bacillus subtilis via a non-classical pathway, exhibiting optimal activity at 55 °C and pH 8.5. Using AlphaFold3 modeling and ΔΔG pred...

High-level surfactin production by Bacillus subtilis through integrated metabolic engineering and bioprocess optimization.

Surfactin is a cyclic lipopeptide biosurfactant produced by Bacillus species and has considerable potential as a sustainable alternative to petroleum-derived surfactants. However, efficient surfactin production remains constrained by the limited productivity of native strains, inadequate precursor supply and suboptimal...

Recent Advances and Future Perspectives in the Biomanufacturing of Lipopeptides: From Strain Engineering to Industrial Applications.