Construction of Specific Parallel Amplification of RNA Ends (SPARE) libraries for the systematic identification of plant microRNA processing intermediates
This method, called SPARE (Specific Parallel Amplific Parallel Amplification of 5 0 RNA Ends) allows the identification of processing intermediates for most of the Arabidopsis miRNAs and enables the determination of the DCL1 processing direction and the cleavage sites introduced by miRNA processing machinery in the precurs...