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N. Gurdo

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Open access Aug 2026

Improving acetate metabolism of P. putida KT2440 by evolutionary and rational engineering

Acetate is a promising carbon source for microbial biotechnology as it can be produced sustainably from lignocellulosic biomass or C1 gases. Since acetate is directly activated to acetyl-CoA, it is especially suitable for producing acetyl-CoA-derived products, showcased here with the production of 3-(3-hydroxyalkanoylo...

M. Filbig, Luisa Wachtendonk, Luca A. I. Hampe et al. · 0 citations
Open access Aug 2026

Adaptive laboratory evolution rewires Pseudomonas putida for resource-efficient acetate assimilation

Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...

N. Gurdo, A. Srinivasan, Tommaso Tagliani et al. · 0 citations

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