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N. Newton

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Open access Aug 2026

Expanding Reverse Genetics of Positive-Strand RNA Viruses: Optimised Rescue Platforms and Construction of a Novel Fluorescent Reporter Nidovirus

It is demonstrated that a potent constitutive promoter system and a hammerhead ribozyme enhance the efficiency of positive-sense RNA virus rescue using CPER, and may support applications requiring efficient recovery of low fitness mutants and diverse mutational libraries and barcoded virus populations.

James R. Potter, Helen Mostafavi, A. Amarilla et al. · 0 citations

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