Construction and Validation of an E. coli Whole-Cell Biosensor Based on CadC and GFP for Heavy Metal Detection.
Whole-cell biosensors provide a cost-effective and sensitive approach for real-time monitoring of toxic metals in environmental samples. In this study, a bacterial whole-cell biosensor was engineered using Escherichia coli BL21(DE3) by integrating the cadC regulatory gene from Bacillus megaterium TWSL_4 with a green fl...