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The discovery of PuYV in bitter gourd, ridge gourd and snake gourd; WGMMV in any cucurbitaceous crop; and ZTMV in snake gourd for the first-time in India are reported.
A complex tomato virome associated with leaf curl disease in Tamil Nadu is revealed, driven by mixed viral infections and recombination and emphasizes the need for continued virome surveillance and integrated disease management in tomato production systems.
Quantitative PCR showed viral accumulation of 105–10⁹ copies/μL, confirming the synergistic role of DNA-B and betasatellites in severe symptom induction and systemic infection, underscoring the complexity of begomovirus pathogenesis.
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