Transcriptional analysis of D, D-carboxypeptidase genes as secondary cephalosporin resistance reporters in Escherichia coli harbouring blaCTX-M.
The emergence of novel plasmid-encoded resistance variants complicates antimicrobial resistance detection by conventional PCR-based methods. Therefore, endogenous secondary resistance genes expressed under antibiotic stresses to produce adaptive resistance mechanisms in bacteria may serve as reporters for AMR detection...