Brain-type creatine kinase (CK-BB) buffers local ATP demand through reversible phosphotransfer between ATP and phosphocreatine, yet how this soluble metabolic enzyme engages membrane compartments is unknown. Here, we combine fluorescence microscopy, DEER spectroscopy, hydrogen–deuterium exchange and native mass spectro...
Samantha Gies, Nolan K. McLaughlin, O. Kou et al.· bioRxiv· 0 citations
α-Synuclein (αSyn) is an intrinsically disordered protein that preferentially binds anionic membranes with lipid packing defects. Cholesterol is an abundant membrane component that regulates packing and organization within membranes, yet its effect on αSyn binding remains unclear, as prior studies report both cholester...
O. Kou, Brian H. Kim, David H. Johnson et al.· Biophysics Reports· 0 citations
α-Synuclein (αSyn) remodels cellular membranes through interactions that involve both its structured, membrane-binding N-terminal domain (NTD) and intrinsically disordered C-terminal domain (CTD). While the amphipathic NTD helix is known to insert into lipid bilayers and generate curvature, the contribution of the acid...
David H. Johnson, Jia Shiuan Liow, O. Kou et al.· Journal of Physical Chemistr...· 0 citations
It is established that αSyn-membrane interactions are governed by a cooperative interplay between membrane phase, curvature, and charge and highlight the importance of resolving both thermodynamic and kinetic contributions to protein-membrane binding.
O. Kou, Cailyn M. Sakurai, Stephanie Y. Ramirez et al.· Langmuir· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
