The complement anaphylatoxin receptor, C3aR, is a prototypical G protein-coupled receptor (GPCR) that plays multi-faceted roles in immune regulation, pro-inflammatory responses, neuroimmune modulation, and host defense mechanisms against pathogens. Recent structural coverage has provided important insights into C3aR ac...
Sudha Mishra, Manish K. Yadav, Annu Dalal et al.· Nature Communications· 1 citation
Abstract CRISPR–Cas12a nucleases typically induce double-strand breaks (DSBs) to initiate genome editing. However, reliance on DSBs risks chromosomal rearrangements and unintended insertions or deletions at off-target sites. Therefore, engineering nickase variants that cleave only a single DNA strand might enable preci...
Ricardo Villegas Warren, Satoshi N. Omura, Diana León-Castro et al.· Nucleic Acids Research· 0 citations
Abstract Cas9 nucleases of CRISPR-Cas adaptive immune systems are programmable RNA-guided DNA endonucleases that evolved from the transposon-associated IscB enzymes. Comparative structural studies have revealed substantial architectural elaboration during this evolutionary transition, including the replacement of the l...
O. Nureki, K. Onishi, Yuta Shuto et al.· Research Square· 0 citations
Cells in multicellular eukaryotic systems are diverse biological units, with characteristics and functions determined by their molecular profiles. CRISPR–Cas9 genome editing has been widely used across biology to modulate gene expression and study gene function. However, there is currently no versatile and scalable met...
Arman Adel, Yuta Shuto, Shunsuke Kawasaki et al.· bioRxiv· 0 citations
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