Open access
Jul 2026
Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases
The present work lays the groundwork for the bio-orthogonal engineering of hydrogenases and related hybrid biocatalysts by employing CRISPR/Cas9-mediated genome editing to integrate a psychrophilic pyrrolysyl-tRNA synthetase/tRNA pair into the Escherichia coli BL21 genome.
Qin Fan, Stevanie Stevanie, S. Frielingsdorf et al.
· Microbial Cell Factories · 0 citations