Global Incorporation of a Photo-Cross-Linking Amino Acid to Probe the Protein Interactome under Physiological Conditions.
Cross-linking mass spectrometry (XL-MS) has advanced as a powerful approach to map protein-protein interactions in cells. With recent advances in cross-linkers, instrumentation, and software, interactions can be observed in a proteome-wide fashion at residue-level resolution. In most XL-MS experiments, a chemical cross...