Process optimization and hybrid nanoflower synthesis of 5- hydroxymethylfurfural oxidase for efficient oxidation of HMF and its derivatives to 2,5- furandicarboxylic acid.
2,5-furandicarboxylic acid (FDCA) is a precursor of the biopolymer Polyethylene furandicarboxylate (PEF). 5-hydroxymethylfurfural oxidase (5-HMFO) oxidizes 5- hydroxymethylfurfural (HMF) to FDCA. One such promising biocatalyst is 5-HMFO from Pseudomonas poae (PSPO). The major limitations of free enzymes include high co...