Multilevel Engineering of Saccharomyces cerevisiae for Scalable Production of Ergosterol as a Vitamin D2 Precursor
Ergosterol, a nutritionally relevant fungal sterol and vitamin D2 precursor, is valuable for food and feed applications, but its overproduction in Saccharomyces cerevisiae is constrained by stringent sterol homeostasis, limited organelle capacity, and the coordination of gene-dense pathways. A multilevel engineering...