While the determination of enzyme kinetic parameters traditionally relies on prolonged and costly experimental methods, machine learning models have recently demonstrated promise as predictive tools. These models still have limitations, such as limited training data and insufficient sensitivity to mutations at import...
Jia-He Qiu, Zong-Ying Lin, Ke-Wei Chen et al.· Journal of Chemical Informat...· 0 citations
The growing global demand for flavor stability and terroir expression in the specialty coffee industry has positioned postharvest fermentation as a core driver of coffee quality upgrading, second in importance only to cultivar genetic improvement. Coffee fermentation is shifting from an empirical spontaneous pectin-rem...
Industrial biocatalysis increasingly requires strategies that convert enzyme discovery into robust catalyst systems for process implementation. Here, we report Spatially Programmable Assembly of Computationally Mined Enzymes (SPACE), a sequence-to-scaffold workflow coupling multiparametric enzyme mining with programm...
Qiang Wang, Teng Bao, Jia-He Qiu et al.· ACS Catalysis· 0 citations
Trehalose is a functional disaccharide widely used in the food, pharmaceutical, and cosmetic industries. It is industrially produced via a dual-enzyme process involving maltoligosaccharide trehalose synthase (MTSase) and maltoligosaccharide trehalose hydrolase (MTHase), with Escherichia coli (E. coli) serving as the ex...
Jianghua Chen, Yu-Jue Wang, Qiang Wang et al.· Fermentation· 0 citations
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