Qianpeng Lai

Creating a non-native bio-amidation activity in asparagine synthetase via biosensor-guided directed evolution for the synthesis of (S)-2-aminobutyramide

The direct asymmetric synthesis of chiral amides remains a focal point in green manufacturing, particularly for (S)-2-aminobutyramide whose traditional production is often hampered by low atom economy and arduous chiral resolution steps. Here, we report the creation of a non-native bio-amidation activity in Escherichia...